Feng X, Cai Y, Dawes K E
Basical Medical Institute, CAMS and PUMC, Beijing.
Zhonghua Jie He He Hu Xi Za Zhi. 1997 Apr;20(2):80-3.
To explore the effect of hypoxia on the proliferation and migration of pulmonary vascular smooth muscle cells (PVSMC), and whether migration of PASMC is involved in the pathogenesis of pulmonary vascular remodeling associated with hypoxia.
In this study, the effect of PDGF, ANP and hypoxia on DNA synthesis and chemotaxis of cultured neonatal calf pulmonary artery smooth muscle cells (PASMC) is investigated by 3H-thymidine incorporation and measurement of cell migration using a 48-well Boyden chamber respectively.
The results demonstrated that hypoxia could stimulate DNA synthesis and chemotaxis of PASMC induced by PDGF; ANP could inhibit DNA synthesis and chemotaxis of PASMC by a cGMP dependent pathway.
It is suggested that PDGF, ANP and hypoxia play important roles in regulating the proliferation and migration of PASMC, which is important in the pathogenesis of hypoxic pulmonary vascular remodeling.
探讨缺氧对肺血管平滑肌细胞（PVSMC）增殖和迁移的影响，以及肺动脉平滑肌细胞（PASMC）的迁移是否参与缺氧相关肺血管重塑的发病机制。
本研究分别采用3H-胸腺嘧啶核苷掺入法和48孔Boyden小室法检测血小板衍生生长因子（PDGF）、心钠素（ANP）和缺氧对培养的新生小牛肺动脉平滑肌细胞（PASMC）DNA合成和趋化性的影响。
结果表明，缺氧可刺激PDGF诱导的PASMC的DNA合成和趋化性；ANP可通过cGMP依赖途径抑制PASMC的DNA合成和趋化性。
提示PDGF、ANP和缺氧在调节PASMC的增殖和迁移中起重要作用，这在缺氧性肺血管重塑的发病机制中具有重要意义。
