Zhu L, Wu J, Liao H, Gao J, Zhao X N, Zhang Z X
School of Medicine, Nanjing University, China.
Zhongguo Yao Li Xue Bao. 1997 Jul;18(4):344-7.
To determine whether the extract of leaves of Ginkgo biloba L (EGb) and several active constituents of EGb have protective effects against glutamate (Glu)-induced neuronal damage.
Microscopy and image analysis of nucleus areas in the arcuate nuclei (AN) of mice were made. The neuronal viability in primary cultures from mouse cerebral cortex was assessed using MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] staining and the intracellular free calcium concentration ([Ca2+]i) of single neuron was measured using Fura-2.
EGb (2.5 mg.L-1) and its constituent ginkgolide B (Gin B, 2 mg.L-1) protected the neuronal viability against Glu-induced injury, and prevented the Glu-induced elevation in [Ca2+]i. EGb (3-10 mg.kg-1) attenuated the decrease of nucleus areas in arcuate nuclei induced by Glu (1 g.kg-1, s.c.).
EGb and Gin B prevent neurons from Glu neurotoxicity through reduction of the rise in [Ca2+]i.
确定银杏叶提取物（EGb）及其几种活性成分是否对谷氨酸（Glu）诱导的神经元损伤具有保护作用。
对小鼠弓状核（AN）的细胞核区域进行显微镜检查和图像分析。使用MTT [3-(4, 5-二甲基噻唑-2-基)-2, 5-二苯基溴化四氮唑]染色评估小鼠大脑皮质原代培养物中的神经元活力，并使用Fura-2测量单个神经元的细胞内游离钙浓度（[Ca2+]i）。
EGb（2.5 mg.L-1）及其成分银杏内酯B（Gin B，2 mg.L-1）保护神经元活力免受Glu诱导的损伤，并防止Glu诱导的[Ca2+]i升高。EGb（3 - 10 mg.kg-1）减轻了Glu（1 g.kg-1，皮下注射）诱导的弓状核细胞核区域的减小。
EGb和Gin B通过降低[Ca2+]i的升高来防止神经元受到Glu的神经毒性作用。
