Monoclonal antibody 3F3 against conformational epitope of Torpedo acetylcholinesterase.
作者信息
Fu F H, Wang Y X, Li F Z, Xin Y B, Sun M J
机构信息
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 May;18(3):284-6.
AIM
To study the type of epitope of native Torpedo acetylcholinesterase (AChE) directed by its monoclonal antibody (McAb) 3F3.
METHODS
Enzyme-linked immunosorbent assay (ELISA) was used for the assay of the reaction between antigen and antibody.
RESULTS
McAb 3F3 immunoreacted well with the native AChE, but not with the reduced- and alkylated-AChE (RA-AChE) at all. Soman did not interfere the binding of 3F3 with AChE molecule. The synthesized 24-peptide containing the active serine residue of the AChE active center did not react with McAb 3F3.
CONCLUSION
3F3 is a monoclonal antibody against the conformational epitope of Torpedo AChE active center, but dose not occupy the active serine residue of the enzyme.
Zotero 插件