Tang Y, Wen B, Guo S, Li X, Yan X
Department of Clinical Laboratory, Xiangya Hospital, Hunan Medical University, Changsha.
Hunan Yi Ke Da Xue Xue Bao. 1997;22(6):533-4.
ATP is a kind of necessary metabolites in the living cells. We detected ATP contents in 222 bacteriuria or the urine germ by bioluminescence method. The results showed that the patient had been infected when the mean value was > 130.9 +/- 11.38 and had not when the mean value was < 32.9 +/- 2.26. The results were the same with the Gram Stain, L-form bacteriuria culture and the common bacteriuria culture. The mean values of positive specimens are higher than that of the negative specimens by the bioluminescence. Thus, it is indicated that the bioluminescence method has high sensitivity. This method can be used to detect L-form bacteriuria in the urine germ quickly and is important for early diagnosis by the doctors.
ATP是活细胞中的一种必需代谢产物。我们采用生物发光法检测了222例菌尿症或尿液细菌中的ATP含量。结果显示,当平均值>130.9±11.38时患者已被感染,而当平均值<32.9±2.26时则未被感染。革兰氏染色、L型菌尿培养和普通菌尿培养的结果与之相同。通过生物发光法检测,阳性标本的平均值高于阴性标本。因此,表明生物发光法具有高灵敏度。该方法可快速检测尿液细菌中的L型菌尿,对医生进行早期诊断具有重要意义。