[Study of detecting p53 gene of laryngeal carcinoma on paraffin-embedded tissue sections by in situ polymerase chain reaction].

In order to investigate alterations of p53 gene in laryngeal carcinoma, five cases of paraffin-embedded tissue were detected by in situ polymerase chain reaction (PCR). The results showed that p53 gene was presented in more than 90% of cancer cells in all cases with exon 5 specific primers, which signal copy numbers were 2.24 +/- 0.23. It suggests that in situ PCR and paraffin-embedded tissue are able to be used in the single copy gene research. The improvement and application of the technique were discussed.