Miller S C, Haberer J, Venkatachalam U, Furniss M J
Pharmaceutical Discovery Division, SRI International, Menlo Park, California 94025, USA.
Radiat Res. 1999 Mar;151(3):310-8.
A number of studies have reported that human leukemia cells respond to exposure to power-line frequency electromagnetic fields (EMFs), providing evidence for an EMF-induced signaling pathway involving activation of protein tyrosine kinases (PTKs), phospholipase-Cy and protein kinase C (PKC). Because activation of PKC is also important in the signaling pathways that regulate the transcription factors NF-kappaB and AP-1, we evaluated the effect of exposure to a 60 Hz EMF on NF-kappaB or AP-1-dependent reporter gene expression in cells of the human promonocytic U937 leukemia cell line. Reporter genes were electroporated into U937 cells and activation of the NF-kappaB or AP-1 signaling pathway was evaluated by measuring chloramphenicol acetyltransferase (CAT) protein by CAT ELISA. In contrast to the effects of well-understood chemical or biological agents, the exposure to magnetic-field intensities of 0.08, 0.1, 1.0 or 1.3 mT had no effect on the NF-kappaB or AP-1 signaling pathways.
多项研究报告称，人类白血病细胞对暴露于电力线频率电磁场（EMF）有反应，这为涉及蛋白酪氨酸激酶（PTK）、磷脂酶-Cγ和蛋白激酶C（PKC）激活的EMF诱导信号通路提供了证据。由于PKC的激活在调节转录因子NF-κB和AP-1的信号通路中也很重要，我们评估了暴露于60 Hz EMF对人原单核细胞U937白血病细胞系细胞中NF-κB或AP-1依赖性报告基因表达的影响。将报告基因电穿孔导入U937细胞，并通过CAT ELISA测量氯霉素乙酰转移酶（CAT）蛋白来评估NF-κB或AP-1信号通路的激活。与已充分了解的化学或生物制剂的作用不同，暴露于0.08、0.1、1.0或1.3 mT的磁场强度对NF-κB或AP-1信号通路没有影响。
