Pal S, Barnhart K M, Wei Q, Abai A M, Peterson E M, de la Maza L M
Department of Pathology, Medical Sciences I, University of California, Irvine 92697-4800, USA.
Vaccine. 1999 Feb 5;17(5):459-65. doi: 10.1016/s0264-410x(98)00219-9.
A DNA plasmid encoding the gene of the major outer membrane protein (MOMP) of the Chlamydia trachomatis mouse pneumonitis (MoPn) serovar and three plasmids containing the variable domains (VD) of the MOMP were constructed. Female mice were inoculated with the plasmids and 60 days later were challenged in the genital tract with C. trachomatis. Six weeks after challenge female mice were caged with male mice and the course of the mating followed. Mice immunized with the MOMP plasmids mounted weak humoral and cell mediated immune responses. However, following the genital challenge no significant differences in vaginal shedding were observed between the groups immunized with the MOMP and control plasmids. In addition, the fertility rates were similar in the experimental and negative control groups. In conclusion, vaccination with DNA plasmids encoding the MOMP elicited a modest immune response but did not protect against infection or disease.
构建了一种编码沙眼衣原体小鼠肺炎（MoPn）血清型主要外膜蛋白（MOMP）基因的DNA质粒以及三种包含MOMP可变区（VD）的质粒。给雌性小鼠接种这些质粒，60天后用沙眼衣原体对其生殖道进行攻击。攻击六周后，将雌性小鼠与雄性小鼠关在同一笼中，并观察交配过程。用MOMP质粒免疫的小鼠产生了较弱的体液免疫和细胞介导免疫反应。然而，在生殖道攻击后，用MOMP质粒免疫的组与对照质粒免疫的组在阴道排菌方面未观察到显著差异。此外，实验组和阴性对照组的生育率相似。总之，用编码MOMP的DNA质粒进行疫苗接种引发了适度的免疫反应，但不能预防感染或疾病。
