Xian H, Liu S, Gu X, Yan Z, Fan M
Institute of Basic Medical Sciences Beijing.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 1997 Aug;13(3):268-70.
The effects of rhCNTF on dorsal root ganglia (DRG) sensory neurons and spinal cord (SC) motor neurons of 10-day chicken embryos were observed with serum-free culture. The results indicated that rhCNTF supported the survival and differentiation of these neurons and showed certain dose-dependent manners. No effect was found at 0.5 ng/ml rhCNTF concentration, a little survival promotive effect at 1.0-1.5 ng/ml, peak effect at 4 ng/ml and no more effects from 8.0 to 100 ng/ml. The survival number of neurons on 7th day was compared, and results showed higher sensitivity of sensory neuron to CNTF shortage than that of motoneuron. This suggests that survival effect of CNTF on motoneuron is only small part of its neurotrophic effects.
采用无血清培养法观察重组人睫状神经营养因子（rhCNTF）对10日龄鸡胚背根神经节（DRG）感觉神经元和脊髓（SC）运动神经元的作用。结果表明，rhCNTF能支持这些神经元的存活和分化，并呈现出一定的剂量依赖性。rhCNTF浓度为0.5 ng/ml时无作用，1.0 - 1.5 ng/ml时有轻微的存活促进作用，4 ng/ml时作用达峰值，8.0至100 ng/ml时无进一步作用。比较第7天神经元的存活数量，结果显示感觉神经元对CNTF缺乏的敏感性高于运动神经元。这表明CNTF对运动神经元的存活作用只是其神经营养作用的一小部分。
