[白细胞介素对培养的大鼠垂体前叶细胞增殖的影响]

[Effect of interleukins on proliferation of cultured rat anterior pituitary cells].

作者信息

Wang G, Zhu Y, Hu Y, Zhang W

机构信息

Department of Physiology, Fourth Military Medical University, Xian.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 1997 Feb;13(1):64-7.

PMID:10074322

Abstract

The present study investigated whether IL-1 and IL-6 involved in the control of proliferation in cultured anterior pituitary (AP) cells. Primary culture of AP cell were obtained from either male and female Sprague-Dawley rats. The effects of IL-1 and IL-6 assessed by [3H]-thymidine incorporation rate. It was found that: (1) IL-1(1-100 ng/ml) stimulated 3H-TdR incorporation into AP cells of male and female rats. (2) The lower concentration of IL-6 (0.1 ng/ml) inhibited 3H-TdR incorporation rate, but higher concentration of IL-6 (1-10 ng/ml) stimulated 3H-TdR incorporation into AP cells of male rats. (3) IL-6 (0.1-10 ng/ml) stimulated 3H-TdR incorporation into AP cells of female rats. These results indicated that IL-1 and IL-6 can modulate the endocrine activity as well as the proliferation of AP cells.

摘要

本研究调查了白细胞介素-1（IL-1）和白细胞介素-6（IL-6）是否参与调控培养的垂体前叶（AP）细胞的增殖。AP细胞原代培养取自雄性和雌性斯普拉格-道利大鼠。通过[3H] - 胸腺嘧啶核苷掺入率评估IL-1和IL-6的作用。结果发现：（1）IL-1（1 - 100 ng/ml）刺激雄性和雌性大鼠AP细胞中3H - TdR的掺入。（2）较低浓度的IL-6（0.1 ng/ml）抑制3H - TdR掺入率，但较高浓度的IL-6（1 - 10 ng/ml）刺激雄性大鼠AP细胞中3H - TdR的掺入。（3）IL-6（0.1 - 10 ng/ml）刺激雌性大鼠AP细胞中3H - TdR的掺入。这些结果表明，IL-1和IL-6可调节AP细胞的内分泌活性以及增殖。