Hu D, Liu H, Ma C
Department of Cardiovascular Physiology, Hu Nan Medical University Changsha.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 1997 Feb;13(1):80-2.
By perfusion with trypsin at low concentration, the intra -pulmonary arterioles were separated carefully and cut into fine tissue pieces. These pieces were then washed with culture medium and used for culture of smooth muscle cells by method of adhesion to wall. Cells cultured were typical smooth muscle cells under light microscope, electron microscope and immunohistochemical test. The method is simple and convenient. It is also applicable to culture of vascular smooth muscle cells from other organs and provides ideal materials for vascular physiopathologic studies.
通过用低浓度胰蛋白酶灌注,小心分离肺内小动脉并切成细小的组织块。然后将这些组织块用培养基洗涤,并采用贴壁法用于平滑肌细胞培养。培养的细胞在光学显微镜、电子显微镜和免疫组织化学检测下均为典型的平滑肌细胞。该方法简单便捷。它也适用于其他器官血管平滑肌细胞的培养,为血管生理病理学研究提供了理想的材料。