Suppr超能文献

[兔肺内小动脉平滑肌细胞的培养]

[Cultivation of intra-pulmonary arteriolar smooth muscle cell from rabbit].

作者信息

Hu D, Liu H, Ma C

机构信息

Department of Cardiovascular Physiology, Hu Nan Medical University Changsha.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 1997 Feb;13(1):80-2.

Abstract

By perfusion with trypsin at low concentration, the intra -pulmonary arterioles were separated carefully and cut into fine tissue pieces. These pieces were then washed with culture medium and used for culture of smooth muscle cells by method of adhesion to wall. Cells cultured were typical smooth muscle cells under light microscope, electron microscope and immunohistochemical test. The method is simple and convenient. It is also applicable to culture of vascular smooth muscle cells from other organs and provides ideal materials for vascular physiopathologic studies.

摘要

通过用低浓度胰蛋白酶灌注,小心分离肺内小动脉并切成细小的组织块。然后将这些组织块用培养基洗涤,并采用贴壁法用于平滑肌细胞培养。培养的细胞在光学显微镜、电子显微镜和免疫组织化学检测下均为典型的平滑肌细胞。该方法简单便捷。它也适用于其他器官血管平滑肌细胞的培养,为血管生理病理学研究提供了理想的材料。

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验