Faniello M C, Bevilacqua M A, Condorelli G, de Crombrugghe B, Maity S N, Avvedimento V E, Cimino F, Costanzo F
Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli 'Federico II', Via S. Pansini 5, I-80131 Napoli, Italy.
J Biol Chem. 1999 Mar 19;274(12):7623-6. doi: 10.1074/jbc.274.12.7623.
We report that the heterotrimeric transcription factor NFY or "CAAT-binding factor" binds the -60 region of the human H ferritin promoter, the B site. DNA binding analysis with specific antibodies demonstrates that NFY/B/C subunits tightly bind this site and that NFY/C subunit is masked in vivo by binding with other protein(s). NFY binds the co-activator p300. Specifically, the NFY/B subunit interacts with the central segment of p300 in vivo and in vitro. cAMP substantially increases the formation of the NFY.p300 complex. Taken together these data provide a general model of cAMP induction of non-CRE-containing promoters and suggest that the NFY-B.p300 complex is located at the 5' end of the promoter and the NFY-B.C. TFIIB on the 3' end toward the transcription start site.
我们报道，异源三聚体转录因子NFY或“CAAT结合因子”结合人H铁蛋白启动子的-60区域，即B位点。用特异性抗体进行的DNA结合分析表明，NFY/B/C亚基紧密结合该位点，并且NFY/C亚基在体内通过与其他蛋白质结合而被掩盖。NFY结合共激活因子p300。具体而言，NFY/B亚基在体内和体外与p300的中央区段相互作用。cAMP显著增加NFY.p300复合物的形成。这些数据共同提供了一个cAMP诱导不含CRE启动子的通用模型，并表明NFY-B.p300复合物位于启动子的5'端，而NFY-B.C.TFIIB位于3'端朝向转录起始位点。
