Cameron F H, Moghaddam M J, Bender V J, Whittaker R G, Mott M, Lockett T J
CSIRO Division of Molecular Science, Sydney Laboratory, 2 Richardson Pl., 103 Delhi Rd, North Ryde, 2113, Sydney, Australia.
Biochim Biophys Acta. 1999 Feb 4;1417(1):37-50. doi: 10.1016/s0005-2736(98)00248-x.
The family of cationic lipid transfection reagents described here demonstrates a modular design that offers potential for the ready synthesis of a wide variety of molecular variants. The key feature of these new molecules is the use of Tris as a linker for joining the hydrophobic domain to a cationic head group. The molecular design offers the opportunity to conveniently synthesise compounds differing in charge, the number and nature of hydrophobic groups in the hydrophobic domain and the characteristics of the spacer between the cationic and hydrophobic moieties. We show that prototype reagents of this design can deliver reporter genes into cultured cells with efficiencies rivaling those of established cationic lipid transfection reagents. A feature of these reagents is that they are not dependent on formulation with a neutral lipid for activity.
本文所述的阳离子脂质转染试剂家族展现出一种模块化设计，这为便捷合成多种分子变体提供了潜力。这些新分子的关键特征是使用三（羟甲基）氨基甲烷（Tris）作为连接基团，将疏水结构域与阳离子头部基团相连。这种分子设计为方便地合成在电荷、疏水结构域中疏水基团的数量和性质以及阳离子和疏水部分之间间隔基团的特性方面存在差异的化合物提供了机会。我们表明，这种设计的原型试剂能够将报告基因导入培养细胞，其效率可与已确立的阳离子脂质转染试剂相媲美。这些试剂的一个特点是它们的活性不依赖于与中性脂质的配方。
