Semenov S N
Institute of Biochemical Physics, RAS, Moscow, Russia.
J Mol Recognit. 1998 Winter;11(1-6):157-62. doi: 10.1002/(SICI)1099-1352(199812)11:1/6<157::AID-JMR413>3.0.CO;2-M.
The possibility was considered to use the transverse thermophoresis of analytes in the capillary for capillary electrophoresis (CE) to control the separation process, decrease the peak width due to thermal effects and provide new separation parameters in CE. As the examination has shown, in non-aqueous buffers the Joule heating in the capillary for CE can provide transverse temperature gradients comparable with the temperature gradients in conventional devices for thermal field flow Fractionation (ThFFF). It was proposed to use the non-uniform velocity profile of analytes caused by the transverse temperature gradient and the temperature dependence of the buffer viscosity for the FFF-like separation of analytes besides CE separation. The expressions for the peak parameters have been derived, where the non-uniform transverse analyte concentration distribution due to the thermophoresis is taken into account, and the possibilities based on FFF-CE principles are discussed. As possible objects of this hyphenated technique, macromolecules and particles are considered.
考虑了利用毛细管中分析物的横向热泳进行毛细管电泳（CE）来控制分离过程、减小热效应导致的峰宽并在CE中提供新的分离参数的可能性。如研究所示，在非水缓冲液中，用于CE的毛细管中的焦耳热可提供与传统热场流分级（ThFFF）装置中的温度梯度相当的横向温度梯度。除了CE分离之外，还提出利用横向温度梯度引起的分析物速度分布不均匀以及缓冲液粘度对温度的依赖性，对分析物进行类似FFF的分离。推导了峰参数的表达式，其中考虑了热泳导致的横向分析物浓度分布不均匀，并讨论了基于FFF-CE原理的可能性。作为这种联用技术的可能对象，考虑了大分子和颗粒。
