Van Regenmortel M H, Altschuh D, Chatellier J, Christensen L, Rauffer-Bruyère N, Richalet-Secordel P, Witz J, Zeder-Lutz G
IBMC, Strasbourg, France.
J Mol Recognit. 1998 Winter;11(1-6):163-7. doi: 10.1002/(SICI)1099-1352(199812)11:1/6<163::AID-JMR414>3.0.CO;2-U.
The introduction in 1990 of a new biosensor technology based on surface plasmon resonance has revolutionized the measurement of antigen-antibody binding interactions. In this technique, one of the interacting partners is immobilized on a sensor chip and the binding of the other is followed by the increase in refractive index caused by the mass of bound species. The following immunochemical applications of this new technology will be described: (1) functional mapping of epitopes and paratopes by mutagenesis; (2) analysis of the thermodynamic parameters of the interaction; (3) measurement of the concentration of biologically active molecules; (4) selection of diagnostic probes.
1990年基于表面等离子体共振的新型生物传感器技术的引入，彻底改变了对抗抗原-抗体结合相互作用的测量。在这项技术中，相互作用的一方固定在传感器芯片上，另一方的结合通过结合物质质量引起的折射率增加来跟踪。将描述这项新技术的以下免疫化学应用：（1）通过诱变进行表位和互补位的功能图谱绘制；（2）分析相互作用的热力学参数；（3）测量生物活性分子的浓度；（4）选择诊断探针。
