Mattiasson B, Kumar A
Department of Biotechnology, Lund University, Sweden.
J Mol Recognit. 1998 Winter;11(1-6):211-6. doi: 10.1002/(SICI)1099-1352(199812)11:1/6<211::AID-JMR425>3.0.CO;2-Y.
Affinity precipitation is fast emerging as a successful technique for the purification of proteins which can be introduced at an early stage of downstream processing. The technique applies the use of reversibly soluble-insoluble polymers which have either natural or synthetic origin. Apart from the successful use of some natural polymers, such as chitosan and alginate, the vast application of the technique depends upon the design of efficient synthetic polymers. In this laboratory, N-isopropylacrylamide (NIPAM) copolymers have been developed for metal chelate affinity precipitation of proteins. The copolymers of 1-vinylimidazole (VI) and iminodiacetic acid (IDA) with NIPAM were synthesized. The copolymers were thoroughly characterized with a view to designing an efficient soluble-insoluble polymer for metal chelate affinity precipitation of proteins.
亲和沉淀作为一种成功的蛋白质纯化技术正迅速兴起，可在下游加工的早期阶段应用。该技术使用具有天然或合成来源的可逆性可溶 - 不溶性聚合物。除了成功使用一些天然聚合物，如壳聚糖和藻酸盐外，该技术的广泛应用取决于高效合成聚合物的设计。在本实验室中，已开发出用于蛋白质金属螯合亲和沉淀的N - 异丙基丙烯酰胺（NIPAM）共聚物。合成了1 - 乙烯基咪唑（VI）和亚氨基二乙酸（IDA）与NIPAM的共聚物。对这些共聚物进行了全面表征，旨在设计一种用于蛋白质金属螯合亲和沉淀的高效可溶 - 不溶性聚合物。
