Toyoda T, Saito S, Inokuchi S, Yabe Y
Department of Orthopaedic Surgery, School of Medicine, Keio University, Tokyo, Japan.
Clin Orthop Relat Res. 1999 Feb(359):221-8. doi: 10.1097/00003086-199902000-00025.
The effect of cyclic tensile load on articular cartilage metabolism was investigated experimentally using 12 Japanese White rabbits. Chondrocytes obtained from the knee joints were cultured on plates with flexible silicone rubber bases. They were subjected to a cyclic (3 seconds on and 3 seconds off) tensile load for 24 hours with a maximum increase in area of 17%. Proteoglycan synthesis, collagen synthesis, and tissue inhibitors of metalloproteinases production by the chondrocytes under the load were quantified and compared with those produced by the control cells in an unloaded condition. The cultured chondrocytes under the cyclic tensile load perpendicularly aligned to the direction of the tensile load. Collagen synthesis and tissue inhibitors of metalloproteinases production increased significantly under the cyclic tensile load, although no significant change in proteoglycan synthesis was observed. These results suggested that the cyclic tensile load on the chondrocytes contribute to the regulation of articular cartilage metabolism in part.
使用12只日本白兔对循环拉伸载荷对关节软骨代谢的影响进行了实验研究。从膝关节获取的软骨细胞在带有柔性硅橡胶基底的培养皿中培养。它们承受循环（3秒加载和3秒卸载）拉伸载荷24小时，最大面积增加17%。对加载条件下软骨细胞的蛋白聚糖合成、胶原蛋白合成以及金属蛋白酶组织抑制剂的产生进行定量，并与未加载条件下对照细胞所产生的进行比较。循环拉伸载荷下培养的软骨细胞垂直于拉伸载荷方向排列。尽管未观察到蛋白聚糖合成有显著变化，但在循环拉伸载荷下胶原蛋白合成和金属蛋白酶组织抑制剂的产生显著增加。这些结果表明，对软骨细胞的循环拉伸载荷部分有助于调节关节软骨代谢。
