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从猪身上分离出的尿路致病性大肠杆菌菌株的毒力相关因子。

Virulence-associated factors of uropathogenic Escherichia coli strains isolated from pigs.

作者信息

de Brito B G, Leite D S, Linhares R E, Vidotto M C

机构信息

Universidade Estadual de Londrina, Departmento de Microbiologia, Pr, Brazil.

出版信息

Vet Microbiol. 1999 Mar 1;65(2):123-32. doi: 10.1016/s0378-1135(98)00277-6.

Abstract

Thirty one Escherichia coli strains isolated from pigs with urinary tract infections were investigated for presence of virulence factors and plasmid DNA profile. The most frequent virulence factors presented by these strains were mannose-resistant fimbriae, including P. fimbriae (54.8%) and aerobactin production (45.2%). The pap) operon, detected by PCR, was found in 54.8% of the strains, which is similar to its frequency in human strains. Other characteristics such as the presence of mannose-sensitive hemagglutinin (16.1%), indicative of type 1 pili, and production of hemolysin (25.8%), colicin (38.7%) and toxins (22.6% for LT and for VT) were less frequent. No strains were positive for STa production. Plasmid profiles were variable among isolates from either the same or different farms.

摘要

对从患有尿路感染的猪身上分离出的31株大肠杆菌菌株进行了毒力因子和质粒DNA图谱分析。这些菌株中最常见的毒力因子是甘露糖抗性菌毛,包括P菌毛(54.8%)和气杆菌素产生(45.2%)。通过PCR检测发现,54.8%的菌株中存在pap操纵子,这与其在人类菌株中的频率相似。其他特征,如甘露糖敏感血凝素的存在(16.1%),表明1型菌毛,以及溶血素的产生(25.8%)、大肠杆菌素(38.7%)和毒素(LT和VT均为22.6%)的频率较低。没有菌株产生STa呈阳性。来自同一或不同农场的分离株之间的质粒图谱各不相同。

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