Gabric N, Dekaris I, Suman L, Karaman Z, Mravicic I
General Hospital 'Sveti Duh', Ophthalmology Department, Zagreb, Croatia.
Exp Eye Res. 1999 Mar;68(3):277-82. doi: 10.1006/exer.1998.0616.
In this study we examined whether immunization with heterotopic corneal graft can be suppressed by usage of cultured corneal tissue. Starting from the hypothesis that the corneal antigenicity might change during long-time storage, we compared, in a mouse model, the immunization obtained with fresh and> stored corneas. Heterotopic (chest wall) mice corneal allografts were exchanged between donors and hosts: (1) mismatched at multiple minor H loci and (2) only H-Y mismatched animals. Median survival time (MST) of primary and secondary skin grafts exchanged between mentioned donors and hosts was recorded. Recipient mice were immunized with either: (a) tail-skin graft, (b) fresh cornea graft or (c) corneal graft stored for three weeks in tissue culture. Three weeks later, recipients were challenged with skin graft placed at the opposite side of the chest wall and MST of these skin grafts was recorded. MST of secondary skin grafts in animals that had been immunized by skin served as a control. In case of multiple minor H disparity, MST of a first-set skin graft was 12 days, as compared to 9 days in case of secondary skin graft (P<0.05). MST of secondary skin graft following immunization by both fresh and stored corneas was 10 days. These data suggest that stored corneas don't loose ability to sensitize the multiple minor H disparate host. It also show that both cultured and fresh corneas, when placed in non-privileged site, have same immunizing capacity as skin (MST of 10 and 9 days, respectively; P>0. 1). When only H-Y disparate animals were used, MST of a first-set skin grafts was 26 days and of secondary skin graft 11 days (P<0.01). In case of H-Y disparity, MST obtained after immunization with fresh and stored corneal tissue (19 and 18 days, respectively) was significantly longer as compared to skin (P<0.05). However, no significant difference in MST of secondary skin grafts between recipients of fresh (19 days) and stored corneal grafts (18 days) was recorded. According to our results, the ability of corneal tissue to immunize both multiple minor H mismatched, as well as only H-Y mismatched host, was not influenced by storage in a tissue culture.
在本研究中，我们检测了使用培养的角膜组织是否能够抑制异位角膜移植免疫。基于角膜抗原性可能在长期储存过程中发生变化这一假设，我们在小鼠模型中比较了新鲜角膜和储存角膜诱导的免疫反应。供体和受体之间交换异位（胸壁）小鼠角膜同种异体移植：（1）在多个次要组织相容性位点不匹配，以及（2）仅存在H-Y不匹配的动物。记录上述供体和受体之间交换的初次和二次皮肤移植的中位存活时间（MST）。受体小鼠分别用以下材料免疫：（a）尾部皮肤移植，（b）新鲜角膜移植，或（c）在组织培养中储存三周的角膜移植。三周后，受体用置于胸壁另一侧的皮肤移植进行攻击，并记录这些皮肤移植的MST。以经皮肤免疫的动物的二次皮肤移植的MST作为对照。在存在多个次要组织相容性差异的情况下，初次皮肤移植的MST为12天，而二次皮肤移植为9天（P<0.05）。经新鲜角膜和储存角膜免疫后的二次皮肤移植的MST均为10天。这些数据表明，储存的角膜不会丧失使多个次要组织相容性不匹配的宿主致敏的能力。这也表明，培养的角膜和新鲜角膜置于非免疫豁免部位时，具有与皮肤相同的免疫能力（MST分别为10天和9天；P>0.1）。当仅使用存在H-Y差异的动物时，初次皮肤移植的MST为26天，二次皮肤移植为11天（P<0.01）。在存在H-Y差异的情况下，经新鲜角膜组织和储存角膜组织免疫后获得的MST（分别为19天和18天）明显长于经皮肤免疫后获得的MST（P<0.05）。然而，新鲜角膜移植受体（19天）和储存角膜移植受体（18天）的二次皮肤移植的MST之间未记录到显著差异。根据我们的结果，角膜组织使多个次要组织相容性不匹配以及仅H-Y不匹配的宿主免疫的能力不受组织培养储存的影响。
