Pongracz J, Lord J M
Medical School, University of Birmingham, Birmingham, B15 2TT, United Kingdom.
Biochem Biophys Res Commun. 1999 Mar 16;256(2):269-72. doi: 10.1006/bbrc.1999.0315.
13-Hydroxyoctadecadienoic acid (13-HODE), hydroxylinoleic acid, is a major lipoxygenase metabolite which is produced by myeloid inflammatory cells and modifies inflammatory cell activity. The biological effects of 13-HODE in non-haemopoietic cells (HUVEC) have been attributed to the incorporation of 13-HODE into 13-HODE containing diacylglycerol and the selective inhibition of protein kinase C. Our studies, using whole promyeloid cells (HL60) and recombinant PKC isoenzymes in an in vitro assay, showed that 13-HODE inhibited PKC-alpha, beta1, and PKC-betall, but did not affect the activity of PKC-delta. These data suggest that the actions of hydroxylinoleic acid on myeloid cells include the selective inhibition of classical PKC isoenzymes.
13-羟基十八碳二烯酸（13-HODE），即羟基亚油酸，是一种主要的脂氧合酶代谢产物，由髓系炎症细胞产生并调节炎症细胞活性。13-HODE在非造血细胞（人脐静脉内皮细胞）中的生物学效应归因于13-HODE掺入含13-HODE的二酰甘油以及对蛋白激酶C的选择性抑制。我们利用全早幼粒细胞（HL60）和重组PKC同工酶进行体外试验的研究表明，13-HODE抑制PKC-α、β1和PKC-βⅡ，但不影响PKC-δ的活性。这些数据表明，羟基亚油酸对髓系细胞的作用包括对经典PKC同工酶的选择性抑制。
