一个与编码主要表面糖蛋白的基因相关的新型卡氏肺孢子虫基因家族。

A new family of Pneumocystis carinii genes related to those encoding the major surface glycoprotein.

作者信息

Schaffzin J K, Sunkin S M, Stringer J R

机构信息

Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0524, USA.

出版信息

Curr Genet. 1999 Mar;35(2):134-43. doi: 10.1007/s002940050442.

DOI:10.1007/s002940050442

PMID:10079332

Abstract

A new family of Pneumocystis carinii genes (called MSR for MSG-related) that encodes peptides related to the major surface glycoprotein (MSG) is described. Members of the MSR sequence family are linked to MSG genes and are located near the ends of at least 13 chromosomes. Transcripts encoding different MSR isoforms were present in a single population of P. carinii f. sp. carinii, showing that multiple MSR genes were expressed. Two size classes of MSR mRNA, 2.4 and 3.5 kb, were detected. Both sizes of MSR mRNA lacked the upstream conserved sequence (UCS), which is found on the 5' end of MSG mRNAs because MSG genes must be linked to the UCS to be transcribed. The absence of the UCS from MSR mRNAs suggests that expression of MSR genes does not require linkage to the UCS locus.

摘要

描述了一个新的卡氏肺孢子虫基因家族（称为与MSG相关的MSR），其编码与主要表面糖蛋白（MSG）相关的肽。MSR序列家族的成员与MSG基因相连，且位于至少13条染色体的末端附近。在单个卡氏肺孢子虫f. sp. 卡氏虫群体中存在编码不同MSR异构体的转录本，表明多个MSR基因被表达。检测到两种大小类别的MSR mRNA，分别为2.4 kb和3.5 kb。两种大小的MSR mRNA均缺乏上游保守序列（UCS），该序列存在于MSG mRNA的5'端，因为MSG基因必须与UCS相连才能被转录。MSR mRNA中不存在UCS表明MSR基因的表达不需要与UCS基因座相连。