Yamamoto S, Sawaguchi S, Fukuchi T, Kanazawa H, Hashizume H, Abe H
Department of Ophthalmology, Niigata University School of Medicine, Japan.
J Cataract Refract Surg. 1999 Mar;25(3):363-7. doi: 10.1016/s0886-3350(99)80084-4.
To examine the 3-dimensional collagen fibrillar architecture of Bowman's layer after radial keratotomy (RK).
Department of Ophthalmology, Niigata University School of Medicine, Niigata, Japan.
This study used monkey eyes in which 0.3 mm deep radial incisions were made on the cornea 2 weeks and 1, 6, and 12 months before the animals were killed. Corneal buttons were immersed in a fixative and the cells macerated with sodium hydroxide 10%. Scanning electron microscopy (SEM) was performed according to standard procedures. A part of the specimens was embedded in epoxy resin for light microscopic (LM) observation for comparison.
The 3-dimensional collagen fibrillar architecture of Bowman's layer was revealed by SEM. The rupture of Bowman's layer could be seen 12 months after surgery and there was no continuity of collagen fibrils in the ruptured area. In LM observations, the width of the stromal incisions gradually became narrower near 12 months after surgery.
Our cell-maceration/SEM method showed that the rupture of Bowman's layer remained up to 12 months after RK. This suggests that discontinuity of Bowman's layer may be responsible for globe rupture after RK.
研究放射状角膜切开术（RK）后鲍曼层的三维胶原纤维结构。
日本新潟大学医学院眼科。
本研究使用在处死动物前2周、1个月、6个月和12个月在角膜上制作0.3毫米深放射状切口的猴眼。将角膜纽扣浸入固定剂中，并用10%氢氧化钠浸软细胞。按照标准程序进行扫描电子显微镜（SEM）检查。将部分标本嵌入环氧树脂中进行光学显微镜（LM）观察以作比较。
通过SEM显示了鲍曼层的三维胶原纤维结构。术后12个月可见鲍曼层破裂，破裂区域的胶原纤维无连续性。在LM观察中，术后12个月左右基质切口宽度逐渐变窄。
我们的细胞浸软/SEM方法显示，RK术后12个月鲍曼层破裂仍存在。这表明鲍曼层的不连续性可能是RK后眼球破裂的原因。
