百日咳博德特氏菌毒力相关外膜蛋白的鉴定

Identification of Bordetella pertussis virulence-associated outer membrane proteins.

作者信息

Passerini de Rossi B N, Friedman L E, González Flecha F L, Castello P R, Franco M A, Rossi J P

机构信息

Departamento de Química Biológica-IQUIFIB, Universidad de Buenos Aires, Argentina.

出版信息

FEMS Microbiol Lett. 1999 Mar 1;172(1):9-13. doi: 10.1111/j.1574-6968.1999.tb13442.x.

DOI:10.1111/j.1574-6968.1999.tb13442.x

PMID:10079522

Abstract

Bordetella pertussis virulence-associated 30-, 32-, 90- and 95-kDa outer membrane proteins were purified and their N-terminal amino acid sequences were determined. The 30- and 32-kDa outer membrane proteins showed identity to the C-terminal region of the precursors of the serum resistance protein (BrkA) and the tracheal colonization factor, respectively. We confirmed the cleavage site of these precursors after N731 for BrkA and after N393 for tracheal colonization factor. Associated with the 32-kDa outer membrane protein, we found a new group of 36-kDa virulence-associated peptides. The 95-kDa outer membrane protein showed identity to Vag8. The 90-kDa outer membrane protein did not show homology with the described proteins. We report the N-termini sequence of Vir-90, a novel potential virulence factor.

摘要

纯化了百日咳博德特氏菌的毒力相关30 kDa、32 kDa、90 kDa和95 kDa外膜蛋白，并测定了它们的N端氨基酸序列。30 kDa和32 kDa外膜蛋白分别与血清抗性蛋白（BrkA）前体的C端区域和气管定植因子具有同一性。我们证实了BrkA前体在N731之后以及气管定植因子在N393之后的切割位点。与32 kDa外膜蛋白相关，我们发现了一组新的36 kDa毒力相关肽。95 kDa外膜蛋白与Vag8具有同一性。90 kDa外膜蛋白与已描述的蛋白没有同源性。我们报告了一种新型潜在毒力因子Vir-90的N端序列。