Fontana C, Favaro M, Frezza D, Favalli C
Department of Experimental Medicine and Biochemical Science, University of Rome Tor Vergata, Italy.
FEMS Microbiol Lett. 1999 Mar 1;172(1):47-52. doi: 10.1111/j.1574-6968.1999.tb13448.x.
The gene encoding for polynucleotide phosphorylase (pnp) of a new biovar of Staphylococcus aureus subsp. aureus (NBSA) has been isolated from a genomic library of strain M280(0). The coding region consisted of a 1094-bp HindIII-HindIII DNA fragment encoding for a protein of 277 amino acids with a calculated molecular mass of 29.5 kDa. The nucleotide sequence of the structural gene, contained a continuous open reading frame of 836 bp, showed significant homology with the genes of bacterial polynucleotide phosphorylase from Bacillus subtilis (67.7% identity), from Haemophilus influenzae (62.4% identity), from Pseudomonas luminescens (61.6% identity), and from Escherichia coli (59.7% identity). DNA-DNA and DNA-colony slot-blot hybridizations demonstrated that the pnp gene, employed as a molecular probe, is specific for the identification of NBSA strains.
已从金黄色葡萄球菌亚种新生物变种（NBSA）菌株M280(0)的基因组文库中分离出编码多核苷酸磷酸化酶（pnp）的基因。编码区由一个1094 bp的HindIII - HindIII DNA片段组成，该片段编码一种277个氨基酸的蛋白质，计算分子量为29.5 kDa。结构基因的核苷酸序列包含一个836 bp的连续开放阅读框，与枯草芽孢杆菌、流感嗜血杆菌、发光假单胞菌和大肠杆菌的细菌多核苷酸磷酸化酶基因具有显著同源性（同一性分别为67.7%、62.4%、61.6%和59.7%）。DNA - DNA和DNA - 菌落斑点杂交表明，用作分子探针的pnp基因对NBSA菌株的鉴定具有特异性。
