[质粒和噬菌体λ DNA 的光致敏失活：8-甲氧基补骨脂素单加合物和双加合物对致死效应的相对贡献及其在 SOS 诱导的大肠杆菌细胞中的修复]
[Photosensitized inactivation of plasmid and bacteriophage lambda DNA: relative contribution to the lethal effect of monoadducts and diadducts of 8-methoxypsoralen and their repair in SOS-induced Escherichia coli cells].
作者信息
Zavil'gel'skiĭ G B, Zavil'gel'skiĭ D G, Sukhodolets V V
机构信息
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
出版信息
Biofizika. 1998 Nov-Dec;43(6):1020-5.
The curves of UV (254 nm)-inactivation and inactivation by furocoumarin derivatives + UVA radiation (PUVA) of bacteriophage lambda and biologically active plasmid pBR322 were measured using Escherichia coli K12 bacteria with different defects of DNA repair system as a ghost. The ratio of mono- and diadducts (interstrand cross-links) of 8-methoxypsoralen was determined that are formed after treating the DNA of pBR322 and bacteriophage lambda with PUVA. It is shown that, on the average, about five monoadducts per one diadduct are formed in DNA of pBR322, and about 0.9 monoadducts per one diadduct are formed in lambda phage DNA. An increased (up to 50%) efficiency of SOS-repair of monoadducts of 8-methoxypsoralen in DNA of pBR322 and lambda in the presence of plasmid pKM101 muc+ (incN) was found.
以具有不同DNA修复系统缺陷的大肠杆菌K12细菌作为宿主菌，测定了噬菌体λ和生物活性质粒pBR322的紫外线（254nm）灭活曲线以及补骨脂素衍生物+紫外线A辐射（PUVA）灭活曲线。测定了用PUVA处理pBR322和噬菌体λ的DNA后形成的8-甲氧基补骨脂素的单加合物和双加合物（链间交联）的比例。结果表明，平均而言，pBR322的DNA中每一个双加合物大约形成5个单加合物，而λ噬菌体DNA中每一个双加合物大约形成0.9个单加合物。发现在质粒pKM101 muc+（incN）存在的情况下，pBR322和λ的DNA中8-甲氧基补骨脂素单加合物的SOS修复效率提高（高达50%）。
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