[来自绿色木霉的真菌天冬氨酸蛋白酶。寡肽水解过程中的特异性]
[Fungal aspartic proteinase from Trichoderma viride. Specificity during oligopeptide hydrolysis].
作者信息
Simankova A N, Mirgorodskaia O A, Savel'eva N V, Savel'ev A N, Kerner R, Rijpstorff P, Alexandrov S L
机构信息
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
出版信息
Bioorg Khim. 1998 Nov;24(11):822-30.
PMID:10079939
Abstract
We isolated, purified, and characterized an aspartic protease from fungus Trichoderma viride. The pH-dependence of the enzyme functioning was determined, and its specificity in the limited proteolysis of insulin and melittin was compared to the specificities of pepsin A and gastricsin. The kinetics of melittin hydrolysis by these enzymes was studied by mass spectrometry.
摘要
我们从绿色木霉中分离、纯化并鉴定了一种天冬氨酸蛋白酶。测定了该酶功能的pH依赖性，并将其对胰岛素和蜂毒肽有限蛋白水解的特异性与胃蛋白酶A和胃泌素的特异性进行了比较。通过质谱研究了这些酶对蜂毒肽的水解动力学。
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