Garcia J J, Bolás-Fernández F, Torrado J J
Dpto. Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Spain.
J Chromatogr B Biomed Sci Appl. 1999 Feb 19;723(1-2):265-71. doi: 10.1016/s0378-4347(98)00497-6.
Three different and complementary chromatographic methods are described for quantitative determination of albendazole (ABZ) and its two main metabolites: albendazole sulphoxide (SOABZ) and albendazole sulphone (SO2ABZ). ABZ, SOABZ and SO2ABZ can be quantified by two RP-HPLC methods with an ODS2 column and two different mobile phases. One of methanol-water (60:40) for ABZ and a second one of phosphoric acid in water-acetonitrile (80:20) for SOABZ and SO2ABZ. SOABZ bears an asymmetric sulphur centre. Quantitative assay of (+) SOABZ and (-) SOABZ can be performed by HPLC. A chiral AGP column and a mobile phase of sodium phosphate buffer (8 mM, pH 7.0) containing different amounts of 2-propanol between 0 to 2% were used. Pharmacokinetic characteristics of ABZ following oral administration of a liquid formulation of ABZ (12 mg/kg) in mice has been studied with these three complementary HPLC methods and the results are reported.
本文描述了三种不同且互补的色谱方法，用于定量测定阿苯达唑（ABZ）及其两种主要代谢物：阿苯达唑亚砜（SOABZ）和阿苯达唑砜（SO2ABZ）。ABZ、SOABZ和SO2ABZ可通过两种使用ODS2柱和两种不同流动相的反相高效液相色谱（RP-HPLC）方法进行定量。一种流动相为甲醇-水（60:40）用于测定ABZ，另一种流动相为水-乙腈（80:20）中的磷酸用于测定SOABZ和SO2ABZ。SOABZ具有一个不对称硫中心。（+）SOABZ和（-）SOABZ的定量测定可通过高效液相色谱法进行。使用了手性AGP柱和含有0至2%不同量异丙醇的磷酸钠缓冲液（8 mM，pH 7.0）作为流动相。用这三种互补的高效液相色谱方法研究了小鼠口服阿苯达唑液体制剂（12 mg/kg）后阿苯达唑的药代动力学特征，并报告了结果。
