Tsuda T, Takino A, Kojima M, Harada H, Muraki K
Shiga Prefectural Institute of Public Health and Environmental Science, Ohtsu, Japan.
J Chromatogr B Biomed Sci Appl. 1999 Feb 19;723(1-2):273-9. doi: 10.1016/s0378-4347(98)00524-6.
A simple and rapid method is described for the GC-MS determination of 4-nonylphenols (NOs) and 4-tert-octylphenol (OC) in biological samples. The NOs and OC in the sample are extracted with acetonitrile and the lipid in the sample extract is eliminated by partitioning between hexane and acetonitrile. After Florisil PR column clean-up, the sample extract is analyzed by GC-MS in the selected ion monitoring (SIM) mode. Average recoveries in pale chub (fish) and corbicula (shellfish) are 86.0 and 93.4% for NOs, and 95.8 and 96.4% for OC, respectively, spiked at the levels of 1.0 microg of NOs and 0.1 microg of OC per 5 g of fish and shellfish samples. The detection limits are 20 ng/g for NOs and 2 ng/g for OC.
描述了一种简单快速的方法，用于气相色谱-质谱联用（GC-MS）测定生物样品中的4-壬基酚（NOs）和4-叔辛基酚（OC）。样品中的NOs和OC用乙腈萃取，样品萃取液中的脂质通过己烷和乙腈之间的分配去除。经弗罗里硅土PR柱净化后，样品萃取液采用气相色谱-质谱联用仪在选择离子监测（SIM）模式下进行分析。在每5克鱼类和贝类样品中分别添加1.0微克NOs和0.1微克OC的水平下，麦穗鱼（鱼类）和蚬（贝类）中的NOs平均回收率分别为86.0%和93.4%，OC的平均回收率分别为95.8%和96.4%。NOs的检测限为20纳克/克，OC的检测限为2纳克/克。
