Nikolić-Vukosavljević D, Grujić-Adanja G, Janković R, Branković-Magić M, Polić D, Jovanović D, Mitrović L
Institute of Oncology and Radiology of Serbia, Belgrade, Yugoslavia.
Tumori. 1998 Nov-Dec;84(6):691-4. doi: 10.1177/030089169808400616.
Knowledge of the steroid hormone receptors has proved to be of significant value in breast cancer. In the present study the possible importance of estrogen-regulated pS2 protein was investigated. Our direct purpose was to answer the question whether the expression of pS2 may be a marker of functional heterogeneity with respect to the steroid hormone receptor status.
The study included 152 patients with primary, operable, histologically confirmed breast carcinomas. Histology specimens were reviewed and classified according to type, nodal status, tumor size and grade. Steroid hormone receptors were assayed by biochemical methods according to the procedures recommended by the EORTC. pS2 protein measurement was performed in breast carcinoma cytosols using an immunoradiometric assay. The results were analyzed by non-parametric statistical methods.
A statistically significant inverse correlation between pS2 protein expression and histological tumor grade was found. The expression of pS2 protein was confirmed to be correlated with steroid hormone receptor status. However, it is important to point out that in spite of these statistically significant findings there were no significant biological associations due to overlapping individual pS2 protein values. The baseline level of expression of pS2 protein was obtained in histological grade III carcinomas with a negative steroid hormone receptor status. It was shown that the distribution of carcinomas according to the baseline level of pS2 protein expression was heterogeneous among estrogen receptor-positive carcinomas, and strikingly homogeneous among estrogen and progesterone-negative carcinoma.
Our study suggested that PR and pS2 protein may identify distinct subsets of estrogen receptor-positive breast carcinomas.
甾体激素受体知识已被证明在乳腺癌研究中具有重要价值。在本研究中，我们调查了雌激素调节的pS2蛋白的潜在重要性。我们的直接目的是回答pS2的表达是否可能是甾体激素受体状态功能异质性的一个标志物这一问题。
本研究纳入了152例经组织学确诊的原发性可手术乳腺癌患者。对组织学标本进行复查，并根据类型、淋巴结状态、肿瘤大小和分级进行分类。按照欧洲癌症研究与治疗组织（EORTC）推荐的程序，采用生化方法检测甾体激素受体。使用免疫放射分析法定量检测乳腺癌细胞溶质中的pS2蛋白。采用非参数统计方法分析结果。
发现pS2蛋白表达与组织学肿瘤分级之间存在显著的负相关。pS2蛋白的表达与甾体激素受体状态相关。然而，必须指出的是，尽管这些结果具有统计学意义，但由于个体pS2蛋白值的重叠，并无显著的生物学关联。在组织学分级为III级且甾体激素受体状态为阴性的癌组织中获得了pS2蛋白的基线表达水平。结果显示，根据pS2蛋白表达的基线水平，癌组织在雌激素受体阳性癌中分布不均一，而在雌激素和孕激素受体均阴性的癌中分布极为均一。
我们的研究表明，PR和pS2蛋白可能可识别雌激素受体阳性乳腺癌的不同亚组。
