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Recognition of sequence-directed structure of the ssDNA backbone by nucleases.

作者信息

Akaboshi E

机构信息

Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita 565, Japan.

出版信息

Biochem Biophys Res Commun. 1999 Mar 24;256(3):532-6. doi: 10.1006/bbrc.1999.0373.

DOI:10.1006/bbrc.1999.0373
PMID:10080932
Abstract

Escherichia coli endonuclease I and exonuclease VII appear to recognize sequence-dependent conformations in the ssDNA backbone. ssDNAs, containing either A- and/or T-tract or a CAP binding region, were digested with these nucleases under conditions which minimize the formation of secondary structures. The digestion patterns were examined in relation to previous results of biochemical and crystallographic studies on dsDNA, and showed broad agreement. Endonuclease I cleaved ssDNA at sites corresponding to bent sites in dsDNA.

摘要

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