Pérez M, Avila J
Centro de Biología Molecular, Universidad Autónoma de Madrid, 28049-, Madrid, Spain.
Biochim Biophys Acta. 1999 Mar 8;1449(2):150-6. doi: 10.1016/s0167-4889(99)00008-7.
Protein phosphatase 2A (PP2A) activity may be differentially regulated by the expression of proteins containing a related amino acid sequence motif such as the casein kinase 2alpha (CK2alpha) subunit or SV40 small t antigen (SVt). Expression of CK2alpha increases PP2A activity whereas SVt decreases its activity. In this work we have tested for the effect of the expression of a third protein containing a similar motif that could be involved in PP2A regulation, the catalytic casein kinase 2alpha' subunit. Our results show that despite the structural similarity of this protein with the other CK2 catalytic (alpha) subunit, the function of the two subunits with respect to the modulation of PP2A activity is quite different: CK2alpha increases whereas CK2alpha' slightly decreases PP2A activity.
蛋白磷酸酶2A（PP2A）的活性可能受到含有相关氨基酸序列基序的蛋白质表达的差异调节，例如酪蛋白激酶2α（CK2α）亚基或SV40小t抗原（SVt）。CK2α的表达增加PP2A活性，而SVt降低其活性。在这项工作中，我们测试了第三种含有类似基序的蛋白质的表达对PP2A调节的影响，该蛋白质为催化性酪蛋白激酶2α'亚基。我们的结果表明，尽管该蛋白质与其他CK2催化（α）亚基在结构上相似，但这两个亚基在调节PP2A活性方面的功能却大不相同：CK2α增加PP2A活性，而CK2α'则略微降低PP2A活性。
