Huang G T, Lee H S, Chen C H, Chiou L L, Lee C Z, Chen D S, Hsu H C, Sheu J C
Department of Internal Medicine, National Taiwan University Hospital, Taipei.
J Formos Med Assoc. 1999 Feb;98(2):92-6.
To better understand the roles of hepatocyte growth factor (HGF) and proliferating cell nuclear antigen (PCNA) in hepatocellular carcinoma (HCC), 37 surgically resected HCCs and corresponding nontumorous liver tissue specimens were collected and the expression of these two factors was quantified by Western blot analysis. Both HGF and PCNA expression levels were significantly higher in tumor tissue than in nontumorous liver tissue. However, their expression levels in HCC tissue and nontumorous tissue did not show any significant correlation with the recurrence of HCC. In addition, HGF and PCNA did not correlate with Edmondson's grade, invasiveness of tumor, presence of tumor capsule, or tumor size. No correlation was found between the expression levels of HGF and PCNA in HCC tissue. We conclude that, although both HGF and PCNA are present at higher levels in HCC tissue than in nontumorous liver tissue, they play little role in the clinicopathologic manifestations of this tumor. HGF appears to contribute little, if at all, to the proliferative activity of HCC cells.
为了更好地理解肝细胞生长因子（HGF）和增殖细胞核抗原（PCNA）在肝细胞癌（HCC）中的作用，收集了37例手术切除的肝癌组织及相应的癌旁肝组织标本，采用蛋白质免疫印迹分析法定量检测这两种因子的表达。结果显示，肿瘤组织中HGF和PCNA的表达水平均显著高于癌旁肝组织。然而，它们在肝癌组织和癌旁组织中的表达水平与肝癌复发无显著相关性。此外，HGF和PCNA与Edmondson分级、肿瘤侵袭性、肿瘤包膜及肿瘤大小均无关。肝癌组织中HGF和PCNA的表达水平之间也无相关性。我们得出结论，尽管HGF和PCNA在肝癌组织中的表达水平均高于癌旁肝组织，但它们在该肿瘤的临床病理表现中作用不大。HGF似乎对肝癌细胞的增殖活性几乎没有贡献。
