Waris T, Reijonen K
Ann Chir Gynaecol. 1976;65(5):328-31.
In order to study the effects of skin freezing in the rat on cutaneous vasculature and adrenergic nerves, a combination of trypan blue and formaldehyde-induced catecholamine fluorescence was used. It was shown that trypan blue clearly demonstrates both hyperaemic and ischaemic areas as well as changes in blood cessel permeability after experimental cold injury. Combination of the two fluorescence methods made it possible to correlate rough changes in blood vessel flow and permeability with changes in adrenergic nerves. Twentyfour hours after freezing of the skin, denervation of the most seriously damaged area was apparent. At the border of the cold injury, signs suggesting accumulation of actecholamines were observed in the subcutaneous nerves.
为了研究大鼠皮肤冷冻对皮肤血管和肾上腺素能神经的影响，采用了台盼蓝和甲醛诱导的儿茶酚胺荧光相结合的方法。结果表明，台盼蓝能清楚地显示实验性冷损伤后充血和缺血区域以及血管通透性的变化。两种荧光方法的结合使得将血管流量和通透性的大致变化与肾上腺素能神经的变化联系起来成为可能。皮肤冷冻24小时后，最严重受损区域的去神经支配明显。在冷损伤边界处，皮下神经中观察到提示儿茶酚胺积聚的迹象。
