Roskell D E, Biddolph S C
University of Oxford Nuffield, Dept. of Pathology and Bacteriology, John Radcliffe Hospital, Oxford OX3 9DU, UK.
Eur J Med Res. 1999 Mar 26;4(3):105-6.
Myxomas are thought to be slowly growing benign neoplasms. Presentation is often due to embolic phenomena, though rapid increase in size is sometimes seen. Such an increase may be due to proliferation of cellular components, an increase in matrix due to synthesis or oedema, haemorrhage into the lesion, or the addition of surface thrombus. Routine microscopy suggests a low proliferation rate. The aim of this study was to investigate cellular proliferation, and to assess its contribution to tumour growth.
The antibodies JC1, Ki67, PC10, and MIB1 were used to make an immunohistochemical assessment of proliferation in five cases of cardiac myxoma.
A significant difference was seen between number and type of cells stained with PC10 and the other markers. Whilst PC10 stained the nuclei of most (60 - 95%) endothelial and stromal cells in all cases, the other markers stained far fewer cells (up to 5%). All markers stained varying numbers of lymphoid cells.
Proliferation in cardiac myxomas is unlikely to be rapid. The widespread positivity for PC10 suggests that PCNA is not a reliable marker in such tissues. Clinical cases in which myxomas have grown rapidly are probably due to changes in intercellular matrix rather than cellular proliferation.
黏液瘤被认为是生长缓慢的良性肿瘤。其临床表现通常归因于栓塞现象，不过有时可见肿瘤大小迅速增加。这种增大可能是由于细胞成分增殖、因合成或水肿导致的基质增加、肿瘤内出血，或表面血栓形成。常规显微镜检查显示增殖率较低。本研究的目的是调查细胞增殖情况，并评估其对肿瘤生长的作用。
使用抗体JC1、Ki67、PC10和MIB1对5例心脏黏液瘤的增殖情况进行免疫组织化学评估。
PC10染色的细胞数量和类型与其他标志物之间存在显著差异。在所有病例中，PC10可使大多数（60 - 95%）内皮细胞和基质细胞的细胞核着色，而其他标志物染色的细胞则少得多（高达5%）。所有标志物均能使不同数量的淋巴细胞着色。
心脏黏液瘤的增殖不太可能迅速。PC10的广泛阳性表明PCNA在此类组织中不是一个可靠的标志物。黏液瘤迅速生长的临床病例可能是由于细胞间基质的变化而非细胞增殖所致。
