Schreier H J, Robinson-Bidle K A, Romashko A M, Patel G V
Center of Marine Biotechnology, The University of Maryland Biotechnology Institute, Baltimore, 21202, USA.
Extremophiles. 1999 Jan;3(1):11-9. doi: 10.1007/s007920050094.
Multicopy plasmids containing the promoter regions for gdh and mlrA genes from Pyrococcus furiosus were propagated in Haloferax volcanii. High-level expression was detected from gdh promoter sequences, with transcription initiating at the same start-site as that found in P. furiosus. For mlrA, several transcripts were detected, with one initiating at the P. furiosus start-site; removal or disruption of the likely P. furiosus boxA element resulted in the disappearance of this transcript, indicating that these sequences were utilized by the H. volcanii RNA polymerase for initiation. This is the first demonstration of the utilization of promoters from a hyperthermophilic archaeon in a mesophilic haloarchaeon and provides further evidence for the unity of transcription processes in the domain Archaea.
含有来自嗜热栖热菌的gdh和mlrA基因启动子区域的多拷贝质粒在嗜盐栖热菌中进行扩增。从gdh启动子序列检测到高水平表达，转录起始位点与嗜热栖热菌中的相同。对于mlrA，检测到多个转录本，其中一个在嗜热栖热菌的起始位点起始；去除或破坏可能的嗜热栖热菌boxA元件导致该转录本消失，表明嗜盐栖热菌RNA聚合酶利用这些序列进行起始。这是首次证明嗜热古菌的启动子在嗜温嗜盐古菌中的利用，并为古菌域中转录过程的统一性提供了进一步证据。
