Nomura M, Ueta Y, Serino R, Yamamoto Y, Shibuya I, Yamashita H
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Neuroendocrinology. 1999 Mar;69(3):167-80. doi: 10.1159/000054416.
The effects of intracerebroventricular (i.c.v.) administration of pituitary adenylate cyclase-activating polypeptide (PACAP) on the expression of c-fos gene as well as heteronuclear (hn) RNA for arginine vasopressin (AVP) in paraventricular (PVN) and supraoptic nuclei (SON) of rats were investigated by immunohistochemistry for c-fos protein (Fos) and in situ hybridization histochemistry for c-fos mRNA and AVP hnRNA. The i.c.v. administration of PACAP (200 pmol/rat) caused a marked induction of Fos-like immunoreactivity (LI) in PVN and SON. The nuclear Fos-LI existed in AVP-LI containing cells in the PVN and SON. The expression of the c-fos gene in the PVN and SON was increased in a dose-related manner 30 min after i.c. v. administration of PACAP. PACAP-induced expression of the c-fos gene was significantly reduced by pretreatment with a PACAP receptor antagonist, PACAP-(6-38)-NH2. In addition, Fos-LI and the expression of the c-fos gene were also observed in the periventricular region of the third ventricle after i.c.v. administration of PACAP. The induction of c-fos gene expression in the PVN and SON reached a maximum 30 min after PACAP administration. The expression of c-fos gene in the PVN and SON induced by i.c.v. administration of vasoactive intestinal peptide (200 pmol/rat) was weaker than that induced by PACAP. The hnRNA for AVP in the PVN and SON was significantly increased 30 min after i.c.v. administration of PACAP (200 pmol/rat). Our results suggest that PACAP activates PVN and SON neurons via PACAP receptors and, in parallel, transcription of the AVP gene in the PVN and SON.
通过对c-fos蛋白（Fos）进行免疫组织化学以及对c-fos mRNA和精氨酸加压素（AVP）核不均一RNA（hnRNA）进行原位杂交组织化学，研究了脑室内（i.c.v.）注射垂体腺苷酸环化酶激活多肽（PACAP）对大鼠室旁核（PVN）和视上核（SON）中c-fos基因表达以及AVP的hnRNA的影响。i.c.v.注射PACAP（200 pmol/大鼠）可引起PVN和SON中Fos样免疫反应性（LI）显著诱导。核Fos-LI存在于PVN和SON中含AVP-LI的细胞中。i.c.v.注射PACAP 30分钟后，PVN和SON中c-fos基因的表达呈剂量依赖性增加。用PACAP受体拮抗剂PACAP-(6-38)-NH2预处理可显著降低PACAP诱导的c-fos基因表达。此外，i.c.v.注射PACAP后，在第三脑室室周区域也观察到Fos-LI和c-fos基因的表达。PACAP给药后30分钟，PVN和SON中c-fos基因表达的诱导达到最大值。i.c.v.注射血管活性肠肽（200 pmol/大鼠）诱导的PVN和SON中c-fos基因表达比PACAP诱导的弱。i.c.v.注射PACAP（200 pmol/大鼠）30分钟后，PVN和SON中AVP的hnRNA显著增加。我们的结果表明，PACAP通过PACAP受体激活PVN和SON神经元，同时，PVN和SON中AVP基因的转录也被激活。
