通过细胞酶联免疫吸附测定法对新的增殖相关蛋白p100进行免疫酶检测：对处于细胞周期S期、G2期和M期的细胞进行特异性检测。

Immunoenzymatic detection of the new proliferation associated protein p100 by means of a cellular ELISA: specific detection of cells in cell cycle phases S, G2 and M.

作者信息

Frahm S O, Rudolph P, Dworeck C, Zott B, Heidebrecht H, Steinmann J, Neppert J, Parwaresch R

机构信息

Department of General Pathology and Hematopathology, University of Kiel, Germany.

出版信息

J Immunol Methods. 1999 Mar 4;223(2):147-53. doi: 10.1016/s0022-1759(98)00217-8.

DOI:10.1016/s0022-1759(98)00217-8

PMID:10089093

Abstract

In vitro proliferation assays are widely used in biomedical research. We describe the immunoenzymatic (ELISA) detection of a recently described proliferation associated protein (p100) by means of a new monoclonal mouse IgG1 antibody (Ki-S2). P100 is a 100 kDa nuclear protein that is specifically detected during the cell cycle phases S, G2 and M. Comparative studies on lectin-stimulated leukocytes using 3H-thymidine labelling and Ki-67 antibodies revealed a statistically significant positive correlation. Since p100 is absent in GO and G1 cells, its detection permits the precise and specific measurement of actual cell cycle events under culture conditions.

摘要

体外增殖试验在生物医学研究中被广泛应用。我们描述了通过一种新的小鼠单克隆IgG1抗体（Ki-S2）免疫酶法（ELISA）检测一种最近描述的增殖相关蛋白（p100）。P100是一种100 kDa的核蛋白，在细胞周期的S期、G2期和M期能被特异性检测到。使用3H-胸腺嘧啶核苷标记和Ki-67抗体对凝集素刺激的白细胞进行的比较研究显示出统计学上显著的正相关。由于GO期和G1期细胞中不存在p100，因此对其进行检测可以在培养条件下精确且特异性地测量实际的细胞周期事件。