灵敏度增强的模拟CT HMQC PFG-HBHA(CO)NH和PFG-CBCA(CO)NH三共振实验。

Sensitivity-enhanced sim-CT HMQC PFG-HBHA(CO)NH and PFG-CBCA(CO)NH triple-resonance experiments.

作者信息

Swapna G V, Montelione G T

机构信息

Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey, 08854-5638, USA.

出版信息

J Magn Reson. 1999 Apr;137(2):437-42. doi: 10.1006/jmre.1998.1699.

DOI:10.1006/jmre.1998.1699

PMID:10089179

Abstract

Short transverse relaxation times of Calpha and Cbeta single-quantum coherences reduce the sensitivity of triple-resonance experiments involving transfers of Calpha/Cbeta or Halpha/Hbeta coherences. Multiple-quantum line-narrowing techniques improve the relaxation properties of 13C coherences, thereby increasing the sensitivity of the experiment. In the present work, we describe PFG-CBCA(CO)NH and PFG-HBHA(CO)NH experiments that utilize heteronuclear multiple-quantum coherences in a simultaneous constant-time period to obtain completely decoupled spectra with improved sensitivity. Results indicate that approximately 30% of cross peaks show an average enhancement of approximately 15% in the CBCA(CO)NH experiment. In the related HBHA(CO)NH experiment, approximately 97% of the cross peaks show an average enhancement of approximately 40%.

摘要

α-碳和β-碳单量子相干的短横向弛豫时间降低了涉及α-碳/β-碳或α-氢/β-氢相干转移的三共振实验的灵敏度。多量子线窄化技术改善了¹³C相干的弛豫特性，从而提高了实验的灵敏度。在本工作中，我们描述了PFG-CBCA(CO)NH和PFG-HBHA(CO)NH实验，这些实验在同时的恒定时段利用异核多量子相干来获得具有更高灵敏度的完全去耦谱。结果表明，在CBCA(CO)NH实验中，约30%的交叉峰平均增强约15%。在相关的HBHA(CO)NH实验中，约97%的交叉峰平均增强约40%。