嗜碱假单胞菌中两种形式的核酮糖-1,5-二磷酸羧化酶/加氧酶的纯化、结晶及初步X射线研究

Purification, crystallization and preliminary X-ray studies of two isoforms of Rubisco from Alcaligenes eutrophus.

作者信息

Hansen S, Hough E, Andersen K

机构信息

Protein Crystallography Group, Department of Chemistry, Faculty of Science, University of Tromso, N-9037 Tromso, Norway.

出版信息

Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):310-3. doi: 10.1107/S0907444998010257. Epub 1999 Jan 1.

DOI:10.1107/S0907444998010257

Abstract

Two different isoforms of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Alcaligenes eutrophus have been purified and crystallized. Both isoforms crystallize in space group P43212. Crystals of isoform I (unit-cell dimensions a = 112.0 and c = 402.7 A) diffract to 2.7 A, whereas isoform II (unit-cell dimensions a = 111.8 and c = 400.0 A) presently diffract to 3.2 A, using synchrotron radiation in both cases.

摘要

已对真养产碱杆菌的两种不同形式的1,5-二磷酸核酮糖羧化酶/加氧酶（Rubisco）进行了纯化和结晶。两种同工型均在空间群P43212中结晶。同工型I的晶体（晶胞尺寸a = 112.0和c = 402.7 Å）衍射至2.7 Å，而异工型II（晶胞尺寸a = 111.8和c = 400.0 Å）目前使用同步辐射在两种情况下衍射至3.2 Å。

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