胡萝卜软腐欧文氏菌胡萝卜软腐亚种内切多聚半乳糖醛酸酶的结晶及初步晶体学分析

Crystallization and preliminary crystallographic analysis of the endo-polygalacturonase from Erwinia carotovora ssp. carotovora.

作者信息

Pickersgill R, Scott M, Smith D, Worboys K, Jenkins J

机构信息

Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ, England.

出版信息

Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):320-2. doi: 10.1107/S0907444998010816. Epub 1999 Jan 1.

DOI:10.1107/S0907444998010816

PMID:10089438

Abstract

Crystals of endo-polygalacturonase from Erwinia carotovora ssp. carotovora have been grown from polyethylene glycol 6000 by the hanging-drop method. Polygalacturonase is important in the virulence of this plant pathogen. The protein crystallizes in space group C2 with unit-cell parameters a = 81.3, b = 53.0, c = 103.1 A, beta = 112.6 degrees and with a single molecule in the asymmetric unit. The crystals diffract to 1.9 A.

摘要

胡萝卜软腐欧文氏菌胡萝卜软腐亚种的内切多聚半乳糖醛酸酶晶体，通过悬滴法从聚乙二醇6000中生长出来。多聚半乳糖醛酸酶在这种植物病原体的致病性中很重要。该蛋白质在空间群C2中结晶，晶胞参数为a = 81.3、b = 53.0、c = 103.1 Å、β = 112.6°，不对称单元中有一个单分子。这些晶体的衍射分辨率达到1.9 Å。