Ahn C S, McMahon T, Sugar J, Zhou L, Yue B Y
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago College of Medicine, 60612, USA.
Cornea. 1999 Mar;18(2):194-8. doi: 10.1097/00003226-199903000-00009.
Levels of alpha1-proteinase inhibitor and alpha2-macroglobulin in the tear film of patients with keratoconus were measured to elucidate their possible roles in the pathogenesis of keratoconus.
Tear samples were collected from 15 keratoconus patients and 14 age-similar human control subjects. Levels of alpha1-proteinase inhibitor and alpha2-macroglobulin in each tear sample were quantified and compared between the two groups.
Mean values for alpha1-proteinase inhibitor were 101.0+/-35.5 and 106.1+/-41.7 ng/microg protein for the keratoconus and control groups, respectively. The corresponding mean values for alpha2-macroglobulin were 13.5+/-6.8 and 14.8+/-7.5 ng/microg protein. Neither inhibitor showed a statistically significant difference between the keratoconus and control specimens. Subset analysis to evaluate the effects of contact lens wear and the presence of a graft in the fellow eye did not reveal a statistically significant difference.
The tear film of patients with keratoconus contains normal levels of protease inhibitors. Therefore, the tear film may not be a source of the reduced inhibitor levels shown in the corneas of patients with keratoconus.
测量圆锥角膜患者泪膜中α1-蛋白酶抑制剂和α2-巨球蛋白的水平，以阐明它们在圆锥角膜发病机制中的可能作用。
从15名圆锥角膜患者和14名年龄相仿的健康对照者中采集泪液样本。对每个泪液样本中α1-蛋白酶抑制剂和α2-巨球蛋白的水平进行定量，并在两组之间进行比较。
圆锥角膜组和对照组α1-蛋白酶抑制剂的平均值分别为101.0±35.5和106.1±41.7 ng/μg蛋白质。α2-巨球蛋白的相应平均值分别为13.5±6.8和14.8±7.5 ng/μg蛋白质。两种抑制剂在圆锥角膜组和对照组样本之间均未显示出统计学上的显著差异。评估隐形眼镜佩戴情况和对侧眼是否存在移植片影响的亚组分析也未发现统计学上的显著差异。
圆锥角膜患者的泪膜中蛋白酶抑制剂水平正常。因此，泪膜可能不是圆锥角膜患者角膜中抑制剂水平降低的来源。
