天蓝色链霉菌A3(2)的ACTVI基因区域参与放线紫红素生物合成中立体特异性吡喃环形成的证据。
Proof that the ACTVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin.
作者信息
Ichinose K, Surti C, Taguchi T, Malpartida F, Booker-Milburn K I, Stephenson G R, Ebizuka Y, Hopwood D A
机构信息
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
出版信息
Bioorg Med Chem Lett. 1999 Feb 8;9(3):395-400. doi: 10.1016/s0960-894x(99)00011-6.
Pyran ring formation in the biosynthesis of actinorhodin in Streptomyces coelicolor A3(2) was studied using the act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the actVI genes. The strain, CH999/pIJ5660 (pRM5 + actVI-ORF1), produced a chiral intermediate, (S)-DNPA, suggesting that the actVI-ORF1 product is a reductase determining the C-3 stereochemical centre.
利用放线紫红素生物合成基因簇（act cluster）缺陷型菌株CH999，该菌株携带基于pRM5的质粒，其中含有actVI基因的组合，对天蓝色链霉菌A3(2)中放线紫红素生物合成过程中的吡喃环形成进行了研究。菌株CH999/pIJ5660（pRM5 + actVI-ORF1）产生了一种手性中间体(S)-DNPA，这表明actVI-ORF1产物是一种决定C-3立体化学中心的还原酶。
Zotero 插件