马传染性贫血病毒的长末端重复序列是细胞嗜性的主要决定因素。

Long terminal repeat sequences of equine infectious anaemia virus are a major determinant of cell tropism.

作者信息

Payne S L, La Celle K, Pei X F, Qi X M, Shao H, Steagall W K, Perry S, Fuller F

出版信息

J Gen Virol. 1999 Mar;80 ( Pt 3):755-759. doi: 10.1099/0022-1317-80-3-755.

PMID:10092016

Abstract

The Wyoming strain of equine infectious anaemia virus (EIAV) is a highly virulent field strain that replicates to high titre in vitro only in primary equine monocyte-derived macrophages. In contrast, Wyoming-derived fibroblast-adapted EIAV strains (Malmquist virus) replicate in primary foetal equine kidney and equine dermis cells as well as in the cell lines FEA and Cf2Th. Wyoming and Malmquist viruses differ extensively both in long terminal repeat (LTR) and envelope region sequences. We have compared the promoter activities of the Wyoming LTR with those of LTRs derived from fibroblast-adapted viruses by examining their abilities to drive a luciferase reporter gene as well as by construction of infectious molecular clones differing only in LTR sequence. Our results indicate that LTR sequences are a major restriction for growth of the Wyoming strain of EIAV in fibroblasts.

摘要

马传染性贫血病毒（EIAV）的怀俄明毒株是一种高毒力的野外毒株，仅在原代马单核细胞衍生的巨噬细胞中能在体外高效复制。相比之下，源自怀俄明的适应成纤维细胞的EIAV毒株（马尔姆奎斯特病毒）能在原代马胎儿肾细胞和马真皮细胞以及FEA和Cf2Th细胞系中复制。怀俄明病毒和马尔姆奎斯特病毒在长末端重复序列（LTR）和包膜区域序列上差异很大。我们通过检测它们驱动荧光素酶报告基因的能力以及构建仅LTR序列不同的感染性分子克隆，比较了怀俄明LTR与源自适应成纤维细胞病毒的LTR的启动子活性。我们的结果表明，LTR序列是EIAV怀俄明毒株在成纤维细胞中生长的主要限制因素。