Chen T, Goldstein J S, O'Boyle K, Whitman M C, Brunswick M, Kozlowski S
Division of Monoclonal Antibodies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda 20892, USA.
Eur J Immunol. 1999 Mar;29(3):809-14. doi: 10.1002/(SICI)1521-4141(199903)29:03<809::AID-IMMU809>3.0.CO;2-X.
T cells play a central role in the initiation, maintenance and regulation of the immune response. Effector responses of T cells are controlled by complex combinations of lymphokines and adhesion/co-stimulatory molecule signals. To isolate the effects of the adhesion/co-stimulatory molecule ICAM-1, we have stimulated purified murine CD4+ and CD8+ T cells with plate-bound anti-CD3 in the presence or absence of plate-bound soluble ICAM-1. In this report, we demonstrate that the co-immobilization of soluble ICAM-1 and anti-CD3 leads to a much greater increase in IL-2 production by CD8+ T cells than CD4+ T cells. The ICAM-1-induced enhancement we observed has differential sensitivity to LFA-1 blockade, depending on the T cell subsets and cytokine evaluated. These effects may play an important role in the generation and modulation of immune responses.
T细胞在免疫反应的启动、维持和调节中发挥核心作用。T细胞的效应反应受淋巴因子和黏附/共刺激分子信号的复杂组合控制。为了分离黏附/共刺激分子ICAM-1的作用，我们在存在或不存在板结合可溶性ICAM-1的情况下，用板结合抗CD3刺激纯化的小鼠CD4+和CD8+ T细胞。在本报告中，我们证明可溶性ICAM-1和抗CD3的共固定导致CD8+ T细胞产生的IL-2比CD4+ T细胞增加得多。我们观察到的ICAM-1诱导的增强对LFA-1阻断具有不同的敏感性，这取决于所评估的T细胞亚群和细胞因子。这些效应可能在免疫反应的产生和调节中发挥重要作用。
