Chistiakova L A, Kravchenko N A, Nikitin S Ia, Iufit S S, Klabunovskiĭ E I
Biofizika. 1976 Nov;21(6):975-9.
Optical density of lysozyme solutions at 290 nm on heating from 20 to 80 degrees C is shown to be changing in multistep way. Character of the changing depends on pH and ionic strength of medium. It is assumed that the results obtained reflect the multistep nature of lysozyme thermal denaturation.
溶菌酶溶液在290纳米处,从20摄氏度加热到80摄氏度时的光密度显示出以多步方式变化。变化的特征取决于介质的pH值和离子强度。据推测,所获得的结果反映了溶菌酶热变性的多步性质。