Van Nassauw L, Tao L, Harrisson F
Acta Histochem. 1999 Feb;101(1):103-12. doi: 10.1016/s0065-1281(99)80010-5.
It is suggested that follicular apoptosis is driven by the status of the BCL-2: BAX rheostat, and that CPP32 is a key effector of granulosa cell death. In the present study, we have immunohistochemically localized two BCL-2 family members, BCL-2 and BAX, and one caspase, CPP32, in the quail ovary during folliculogenesis. BCL-2 was predominantly found in the granulosa cells of developing follicles. BAX was detected in some follicular cells of atretic follicles, and in the nucleus of some prelampbrush oocytes. Expression of CPP32 was detected in leukocytes and in follicular cells of atretic follicles. Immunostaining was also found in interstitial cells, in surface epithelial and vascular endothelial cells, and in some thecal cells of post-ovulatory follicles. In the granulosa cells of non-growing and small prehierarchal follicles, a weak immunostaining was observed. We can conclude that in the avian ovary, BAX and CPP32 are involved in atresia. The present results support the BCL-2: BAX rheostat hypothesis.
有人提出，卵泡凋亡是由BCL-2:BAX恒阻器的状态驱动的，并且CPP32是颗粒细胞死亡的关键效应物。在本研究中，我们通过免疫组织化学方法在鹌鹑卵泡发生过程中的卵巢中定位了两个BCL-2家族成员BCL-2和BAX，以及一种半胱天冬酶CPP32。BCL-2主要存在于发育卵泡的颗粒细胞中。BAX在闭锁卵泡的一些卵泡细胞以及一些灯刷前期卵母细胞的细胞核中被检测到。CPP32的表达在白细胞和闭锁卵泡的卵泡细胞中被检测到。在间质细胞、表面上皮和血管内皮细胞以及排卵后卵泡的一些卵泡膜细胞中也发现了免疫染色。在未生长和小的前等级卵泡的颗粒细胞中，观察到弱免疫染色。我们可以得出结论，在鸟类卵巢中，BAX和CPP32参与闭锁过程。目前的结果支持BCL-2:BAX恒阻器假说。
