Redruello B, Valdes E, Luz Lopez M, Rodicio R
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Oviedo, Spain.
FEBS Lett. 1999 Feb 26;445(2-3):246-50. doi: 10.1016/s0014-5793(99)00120-9.
The ACR1 gene, encoding a succinate-fumarate transporter, is required by the yeast Saccharomyces cerevisiae for ethanol utilization. Accordingly, gene expression is induced by ethanol and repressed by glucose. Here, we investigated three carbon source response elements present in its promoter region. Specific deletions as well as functional analysis of the elements in a heterologous promoter confirmed their role in transcriptional regulation. Protein binding to carbon source response elements of the ICL1 promoter was competed by all three elements to various extents by the respective ACR1 sequences. In addition, two putative stress response promoter elements present in the ACR1 promoter were investigated in deletion analyses and shown to contribute to gene expression.
编码琥珀酸-延胡索酸转运蛋白的ACR1基因是酿酒酵母利用乙醇所必需的。因此，该基因的表达受乙醇诱导，受葡萄糖抑制。在此，我们研究了其启动子区域中存在的三种碳源反应元件。在异源启动子中对这些元件进行特异性缺失以及功能分析，证实了它们在转录调控中的作用。与ICL1启动子的碳源反应元件结合的蛋白质，在不同程度上被相应的ACR1序列中的所有三种元件竞争。此外，对ACR1启动子中存在的两个假定的应激反应启动子元件进行了缺失分析，结果表明它们对基因表达有贡献。
