Law S W, Burton D N
Can J Microbiol. 1976 Dec;22(12):1716-9. doi: 10.1139/m76-253.
Fractionation of total lipid extracted from Achlya sp. at various stages of its developmental cycle revealed that in spores total lipid was composed of 62% neutral lipid, 13% phospholipid, and 25% glycolipid. After germination, the proportion of neutral lipid rose slightly after 2 h then fell sharply to 10% after 8 h, whereupon it rose to 55% of total lipid after 30 h of growth, when sporulation was completed. Conversely, phospholipid rose to 77% of total lipid after 8 h, then declined to 40% after 30 h. Glycolipid was maintained at 10-20% of total lipid throughout the life cycle after spore germination. Quantitative determination of neutral lipid components by photo-reflectometry showed that triglycerides accounted for 20% of neutral lipid in spores, and free fatty acids made up 50%. During growth, the absolute levels of both components fell precipitously on germination, remained at low levels throughout vegetative growth, and rose at the time of sporulation. The fatty acid composition of total lipid, phospholipid, neutral lipid, and free fatty acid fractions extracted from vegetative and sporulating Achlya cells was determined. The principal fatty acids present in all fractions of both stages of the life cycle were hexadecanoic and octadecanoic acids. Hydroxyhexadecanoic acid, eicosatetraenoic acid, and an unidentified long-chain acid were completely absent from the phospholipids of vegetative cells, although they were found in significant quantities in lipid fractions from other stages of growth.
对从不同发育阶段的绵霉属物种中提取的总脂质进行分级分离，结果显示，在孢子中，总脂质由62%的中性脂质、13%的磷脂和25%的糖脂组成。萌发后，中性脂质的比例在2小时后略有上升，然后在8小时后急剧下降至10%，在生长30小时完成孢子形成时，又上升至总脂质的55%。相反，磷脂在8小时后升至总脂质的77%，然后在30小时后降至40%。孢子萌发后的整个生命周期中，糖脂维持在总脂质的10%-20%。通过光反射法对中性脂质成分进行定量测定表明，甘油三酯在孢子的中性脂质中占20%，游离脂肪酸占50%。在生长过程中，这两种成分的绝对水平在萌发时急剧下降，在整个营养生长阶段保持在低水平，并在孢子形成时上升。测定了从营养期和孢子形成期的绵霉细胞中提取的总脂质、磷脂、中性脂质和游离脂肪酸部分的脂肪酸组成。生命周期两个阶段所有部分中存在的主要脂肪酸都是十六烷酸和十八烷酸。羟基十六烷酸、二十碳四烯酸和一种未鉴定的长链酸在营养细胞的磷脂中完全不存在，尽管它们在其他生长阶段的脂质部分中大量存在。
