Wolff D F, Alvarez O A, Vargas F F
J Membr Biol. 1976;30(3):197-202. doi: 10.1007/BF01869668.
The permeability of isolated muscle fibers of the giant barnacle Megabalanus psittacus to water and nonelectrolytes was studied by determining their reflection and permeability coefficients. Reflection coefficients were obtained by comparing the osmotic fluxes produced by a test molecule to that produced by the impermeant sucrose molecule. Permeability coefficients were determined for measurements of tracer uptake. The results indicate that, in these fibers, nonelectrolyte permeability is closely related to lipid solubility and molecular size. A value of 2.16 X 10(-12) cm3/sec dyne for the hydraulic conductivity and a value of 10.45 X 10(-4) cm/sec for 3HHO permeability coefficient were obtained. The effect of membrane surface invaginations and clefts on the determination of permeability coefficients is discussed.
通过测定反射系数和渗透系数,研究了巨型藤壶鹦鹉巴兰巨螯(Megabalanus psittacus)分离肌肉纤维对水和非电解质的渗透性。反射系数是通过比较测试分子产生的渗透通量与非渗透性蔗糖分子产生的渗透通量而获得的。渗透系数通过示踪剂摄取测量来确定。结果表明,在这些纤维中,非电解质渗透性与脂溶性和分子大小密切相关。获得了水力传导率为2.16×10⁻¹² cm³/秒·达因,³HHO渗透系数为10.45×10⁻⁴ cm/秒的值。讨论了膜表面内陷和裂隙对渗透系数测定的影响。