Yatskievych T A, Pascoe S, Antin P B
Department of Nutritional Sciences, University of Arizona, Tucson 85721, USA.
Mech Dev. 1999 Jan;80(1):107-9. doi: 10.1016/s0925-4773(98)00204-4.
Whole mount in situ hybridization studies were performed to investigate the expression pattern of the homeobox gene Hex (also known as Prh) during early stages of chick embryogenesis. At the time of laying, cHex transcripts are detected in Koller's sickle and the forming hypoblast. During gastrulation (HH stage 4), cHex is expressed in anteriorly-displaced hypoblast cells. At stage 6, cHex transcripts are observed within endoderm in an anterior are that overlaps the cardiogenic region. Later cHex expression is observed within pharyngeal endoderm immediately adjacent to the forming myocardium, in the endocardium and in the liver and thyroid gland primordia. cHex transcripts are also detected within blood islands beginning at stage 4, and in extraembryonic and intraembryonic vascular endothelial cells as vessels form.
进行了全胚胎原位杂交研究，以调查同源框基因Hex（也称为Prh）在鸡胚胎发育早期阶段的表达模式。在产卵时，在科勒镰刀区和形成的下胚层中检测到cHex转录本。在原肠胚形成期（HH第4阶段），cHex在前移的下胚层细胞中表达。在第6阶段，在内胚层前部与心脏发生区域重叠的区域观察到cHex转录本。后来，在紧邻形成的心肌的咽内胚层、心内膜以及肝脏和甲状腺原基中观察到cHex表达。从第4阶段开始，在血岛中也检测到cHex转录本，并且在血管形成时在胚外和胚内血管内皮细胞中也检测到cHex转录本。
