Boot R, Thuis H C, Reubsaet F A
Section of Laboratory Animal Microbiology, National Institute of Public Health and the Environment, Bilthoven, The Netherlands.
Zentralbl Bakteriol. 1999 Feb;289(1):9-17. doi: 10.1016/s0934-8840(99)80116-9.
We studied the cellular fatty acid composition of 10 Actinobacillus (A.) and Pasteurella (P.) reference strains grown on 2 types of agar by the MIDI Microbial Identification System (MIS). A. capsulatus, A. equuli, A. lignieresii, A. ureae, A. dagmatis, P. gallinarum, P. haemolytica, P. multocida, P. pneumotropica biotypes Heyl and Jawetz were grown on GC agar supplemented with ascitic fluid and X and V factor (Levinthal's agar = LA agar) or GC agar supplemented with vitox and hemoglobin (VH agar) on 3 to 7 and 7 to 16 occasions respectively and fatty acid methylester (FAME) profiles were submitted to principal component analysis (PCA). All Pasteurellaceae strains showed FAME profiles typical for the family. Maximum coefficients of variation of the percentage of the 3 major FAMEs 14:0, 16:0, and 16:1 cis were 0.03, 0.03 and 0.03 for Pasteurellaceae strains grown on VH agar and 0.09, 0.17 and 0.09 respectively for strains grown on LA agar. PCA of FAME profiles obtained with growth from LA agar generally did not allow species separation of the Pasteurellaceae but most species were clearly discriminated by PCA when they were grown on VH agar. Our findings indicate that the growth medium had a significant effect on the reproducibility of fatty acid profiling in Pasteurellaceae and that PCA of fatty acid data obtained under standardized growth conditions may discriminate Pasteurellaceae species.
我们通过MIDI微生物鉴定系统（MIS）研究了在2种琼脂上生长的10株放线杆菌属（A.）和巴斯德氏菌属（P.）参考菌株的细胞脂肪酸组成。荚膜放线杆菌、马驹放线杆菌、里氏放线杆菌、脲放线杆菌、达氏放线杆菌、鸡巴斯德氏菌、溶血巴斯德氏菌、多杀巴斯德氏菌、嗜肺巴斯德氏菌Heyl和Jawetz生物型分别在补充有腹水以及X和V因子的GC琼脂（Levinthal琼脂=LA琼脂）或补充有维他霉素和血红蛋白的GC琼脂（VH琼脂）上培养，培养次数分别为3至7次和7至16次，然后将脂肪酸甲酯（FAME）谱进行主成分分析（PCA）。所有巴斯德氏菌科菌株均显示出该科典型的FAME谱。对于在VH琼脂上生长的巴斯德氏菌科菌株，3种主要FAME（14:0、16:0和16:1顺式）百分比的最大变异系数分别为0.03、0.03和0.03，而对于在LA琼脂上生长的菌株，分别为0.09、0.17和0.09。从LA琼脂上生长获得的FAME谱进行PCA分析通常无法区分巴斯德氏菌科的物种，但当它们在VH琼脂上生长时，大多数物种通过PCA可以清晰区分。我们的研究结果表明，生长培养基对巴斯德氏菌科脂肪酸谱分析的重现性有显著影响，并且在标准化生长条件下获得的脂肪酸数据进行PCA分析可能会区分巴斯德氏菌科的物种。
